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A influência da dose de antígeno empregada sobre os parâmetros da linha de regressão sôro-complemento poude ser apreciada e dada uma correta interpretação.\r\n6 - A dose de antígeno a ser usada numa titulagem de sôro é aquela que não afeta o relativo paralelismo das curvas de isofixação. Então, complemento fixado será função linear da quantidade de sôro presente.\r\n7 - A parte da curva de isofixação que liga os dois ramos assintóticos, traduz a suplência observada entre sôro e antígeno e indica as condições em que a fixação do complemento depende tanto da quantidade de sôro presente, como da quantidade de antígeno usado. Nesse caso a titulagem de qualquer um desses elementos não poderá ser feita. '\r\n8 - Para a titulagem de soros, por método simplificado, fatores de conversão são utilizados. Esses fatores foram determinados para o sistema lepra onde os valores de h sofrem marcada influência da quantidade de complemento necessário para 50% de hemó1ise, pois há estreita dependência entre a percentagem de complemento fixado e a inclinação da linha de regressão logaritmo de complemento contra logitos de hemólise.\r\n9 - A padronização da reação de fixação do complemento exige a reprodutibilidade das partidas de antígenos. 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